What patients have learned about saliva testing

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The information on Stop The Thyroid Madness is excellent for saliva testing and what we share is generally very consistent with this information.

This is the page about saliva testing on STTM: https://stopthethyroidmadness.com/saliva-testing/


Please take careful note of the labs patients have felt are not doing the trick below for various reasons for our needs as thyroid / adrenal fatigue patients . It’s always up to you, this is just based on patient experience, not medical professionals.

Patients in the UK say that Medichecks isn’t perfect for their needs, but have said they usually get enough info to make it worthwhile, but it’s up to you.

As of 5/2020 patient are reporting that Genova is coming back with many high to VERY high results on MOST tests for MOST persons.  People are saying this doesn’t make sense compared to their history and symptoms.  Up to you to decide if this works and make sense for your situation, this is simply a report of what people have told us in their opinion about their results.

This page is good to study before testing:
Supplements and Meds Influencing Your Cortisol Saliva Results 

Patients report optimal test times (modify is schedule is atypical):

Within 1/2 hour of waking, noon, 4-5 and bedtime.

Labs often seem to suggest no food 2 hours prior to samples.

Patients report:

No caffeine the day of or day before the saliva test (wean to avoid headaches)

Test on as normal of a day as possible (no fights or added stress)

No exercise on test day.

No lip products the day of testing.

Nothing in mouth 1/2 hour before each sample

Thyroid meds taken as normal (Adjusted to not have anything in mouth 1/2 hour prior to each sample)




For support from your fellow patients, please join Adrenal Fatigue and Thyroid Care by clicking here.

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(links are below)

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For those that prefer to order from amazon, here is the link. (it is slightly more costly than other options, but some prefer amazon):

For California and most other states if the Amazon link is out of stock, please check these sites:

Canary club: https://www.canaryclub.org/home-diagnostic-tests/focused-test-profiles/focused-tests/cortisol-4x-stress-profile 

Testing VIA BLT – All states that allow self-testing:


Canada Testing:

Many are using ZRT or Rocky Mountain.
My MedLab ships to Canada.  Can use this number and tell them you saw this via STTM: 417-782-6169

Australia testing:


United Kingdom

Comprehensive Adrenal Stress Test.
This test also measures levels of Secretory IgA an important marker for immune function and helps to show how stress may be impacting on immune function.

Saliva testing WITH Sex Hormones: 
(Note: Some patients report feeling blood testing for sex hormones is more accurate, others report that saliva has been fine for them)  This kit is for sex hormones and cortisol:

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33 Replies to “What patients have learned about saliva testing”

  1. What to start with? Thought I started with Fibromyalgia in early 1990’s. Now think it was hypothyroid, adrenal fatigue, etc. Little to no treatment from doctors. Now have osteoporosis, high blood pressure,etc. Sure, after reading your pages, it all stems from thyroid and lifestyle that exhausted my adrenals. Graduated nursing school age 40 . Mental problems with husband, three kids to raise, worked in intensive care burn center 12 hour shifts. Did all jobs Really well. Exhausted me finally. Where to start to get some life back. In bad shape . Where to find a doctor to help. Too tired to do a lot to help myself now. 1000’s spent on books, supplements,etc. This site has most knowledge I have come across anywhere. What test or tests to get started. Thanks.

  2. the order button (red) does not seem to work as a link, and the amazon link says that the product is unavailable. Please Help!

    1. Looks below it, there are a couple other links. We know Amazon has been out of stock sadly.

  3. There is no reason to freeze and mail over night. Several studies have shown cortisol saliva is extremely resilient in heat and cold and it doesnt not effect results.

    1. This is counter to strong experience that has shown over and over that samples are affected by the heat. It’s your choice what to do however.

    2. Where did you hear that? I don’t think that’s true. Sounds like you’d fudge up other people’s results with no information to back that up.

  4. Do you really have to do this within 30 minutes of waking up? I am one of those people STTM describes as waking up frequently. I have gone off all forms of caffeine and any supplements that would have interfered and it is throwing my sleep off even more.

    If I lie there half dazed for an hour at 7am is it OK to do the saliva test at 8am?

    1. All the optimal values were developed with people testing at waking. So normally people test within 1/2 at the latest.

  5. What about if you are taking hydrocortisone for adrenals? It was suggested to me that I stop it two weeks before testing. Doing that scares the holy crap out of me. Would it even be safe to do that?

    1. I take hydrocortisone and now have low TSH. Just getting started with doctors. Have you found anything out about how hydrocortisone for adrenals affects treatment for Thyroid? Please email anything you know.

  6. Above says that Labcorp , Quest and others 24 hour saliva test is worthless. Can someone elaborate a little on this?

    1. They don’t use full ranges or use less than / greater than signs, so you can’t read the results accurately.

    1. It’s a kit from ZRT labs, which is a good lab. Patients have stressed how important it is to make sure to follow the direction on this and the linked pages to get accurate results and not just follow what the lab says.

  7. Filled tubes mid last week and put in the freezer. Decided to wait till monday to ship. I saw somewhere that we were supposed to ship on ice but I can’t find that info now. Should I ship on dry ice? It’s been 104 here.

    1. That’s really warm weather. We do know that heat can affect the samples. Is there any chance it might cool down if you held the samples for a week or something?

  8. If things cool down, this time of year wed’d be looking at maybe 96 at best. Ugh, I know! Do you think dry ice would be ok?

    1. I don’t know of why dry ice would be a problem but I don’t know of anyone that’s done it.

  9. If doing the first sample 30 min after waking…. If i wake up at 5 and can’t fall back to sleep do I need to do my first sample then or when I get out of bed at 7? And if I need to do it at 5 am do I do all the others in 4 hours intervals? If so, the last sample would be done way before bedtime…
    Thank you!

    1. We have figured out optimal values by testing as close to waking as possible. Not when we got out of bed.

      We always want that last sample when our body is clearly telling us it’s time to sleep.

    1. Anything that is put on the lips that couple contaminate the sample. Chapstick, lipsticks, oils, etc.

  10. Do you know how long it takes to hear results from ZRTLAB? I sent my kit back over 2 weeks ago and have not heard anything. Do they send the results or put online?

    1. Usually about 10 days. I would contact them if you still haven’t heard back. Sorry i didn’t see this a few days ago. Thanks

  11. I just drank a quarter bottle of bai which contains green coffee bean extract And coffee fruit extract. Does that exclude me from taking the four-point cortisol test tomorrow? I know it says no caffeine but I took that just to mean regular coffee or chocolate or tea or sodas.

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